Relationship and prognosis of hospho-Akt and CD44v6 in breast cancer

Abstract

1126 Background: The purpose of this study was to investigate the relationship between pAkt and CD44v6 expression of breast cancer after surgery. We also evaluated the expression patterns and predictive value of pAKT and CD44v6 in breast cancer tissues. Methods: Expression of pAkt and CD44v6 protein was detected using immunohistochemistry in 98 patients. The correlation between the expression of pAkt, CD44v6, and other disease-related characteristics with treatment outcome was investigated. Kaplan-Meier survival analysis and Cox regression were used to observe the correlation between variables and the prognostic value. Results: Of all these 98 cases, 37 cases (37.8%) were observed as positive for pAkt expression, and there were 25 cases (51.0%) pAKT positive expression in the lymph node (+) and 15 (30.6%) in lymph node (-) of 49 cases. pAkt expression had no significant difference in node-positive group and node-negative group. We examined the relationship between pAKT and HER2 expression, pAkt expression was positively correlated with HER-2 expression (p = 0.009). A statistically significant difference in positive staining for pAkt was observed between CD44V6-positive and CD44V6-negative tumors (p = 0.016). CD44v6 positive tumors showed a statistically very highly significant association between node-positive and node-negative (p = 0.013). For prognosis value, a week relationship was observed between pAkt and OS, but DFS was inversely associated with pAkt staining intensity (p = 0.061, p = 0.004). There had a statistical correlation between CD44v6 overexpression and OS and DFS (p = 0.026 and 0.006 respectively). Univariate analysis revealed that lymph node status and CD44V6 were significantly associated with decreased OS and DFS, but there had no significant difference between variables and OS and DFS by multivariate analysis. Conclusions: Our data suggested that there had a intensive relationship between pAKT and CD44v6 expression in breast cancer tissues. We demonstrated that both pAkt and CD44v6 were significantly assiociated with a decreased OS and DFS in breast cancer patients. pAKT and CD44v6 overexpressed in cancer cells may be sensitive markers for predicting the treatment outcome in breast cancer patients. No significant financial relationships to disclose.