Abstract
The use of agro industrial by-products like cassava starch and sugar cane bagasse show great potential as substrates and support amylases production by solid-substrate fermentation (SSF). The production of amylolytic enzymes by Aspergillus niger LPB 28 increased with forced air using a column fermentation system and optimized conditions in a column bioreactor when it was followed by kinetics of respiratory metabolism. The O2 consumption and CO2 production by A. niger were measured in the top of the columns through the use of a GC system linked to a program for chromatograph control and integration with 2m length, with helium as the carrier gas and a thermal conductivity detector. The kinetic parameters were determined using the program FERSOL.
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