Relation between renal and hepatic excretion of drugs: IV. Influence of nephrectomy or bile duct ligation on the accumulation of phenol red in kidney and liver tissue of rats of different ages

Abstract

Based on in-vivo experiments investigating the relation between renal and hepatic excretion of phenol red in rats of different ages it was the aim of the present study to characterize the in-vitro accumulation of this dye in 10- and 55-day-old rats. Furthermore the influence of unilateral (uNX) or bilateral nephrectomy (NX) or bile duct ligation (DL) on renal and/or hepatic accumulation was characterized. The aerobic accumulation of phenol red is nearly twofold in kidney slices compared to that of liver tissue. In both organs and both age groups a self depression limit of phenol red accumulation can be measured. Under anaerobic incubation conditions in the liver nearly no accumulation of the dye occurs. However, in kidney slices the uptake under nitrogen atmosphere surpasses the aerobic accumulation. For this reason the accumulation in kidney slices is mainly a passive one caused by intracellular binding of the dye. There exist an influx carrier system as well as an efflux carrier in the kidney. The hepatic accumulation is first of all an active process. The accumulation capacity develops with increasing age. However, only quantitative differences occur and accumulation of young and adult animals does not differ qualitatively. 24 h after NX or DL and uNX phenol red accumulation is diminished in the liver or in kidney and liver, respectively. Transport capacity of liver tissue is more inhibited after these operations in adult animals than in 10-day-old-rats.