Abstract
Oxygen consumption rates by human spermatozoa in semen and in sugar free medium, and of seminal plasma, were determined. Human spermatozoa were shown to exhibit marked endogenous as well as exogenous respiration. In the absence of extracellular substrates, that is in suspensions of washed spermatozoa, the motility of human spermatozoa is supported solely by endogenous respiration. Removal ofoxygen from the surrounding medium inactivates the spermatozoa and re-aeration restores their motility. Anaerobically, human spermatozoa are able to maintain their motility only at the expense of their exogenous metabolism.
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