Abstract
Background:The role of microRNAs (miRNAs) in peritoneal transport is uncertain.Methods:We studied 82 new peritoneal dialysis (PD) patients, 22 prevalent patients without ultrafiltration problem, and 6 patients with documented ultrafiltration problem. Peritoneal transport was determined by standard peritoneal equilibration test (PET). RNA was extracted from the PD effluent after PET, and intra-peritoneal expression of miRNA targets were quantified.Results:There were significant difference in the PDE expressions of miR-15a and miR-21. There were modest inverse correlations between ultrafiltration volume and PDE expression of miR-17 (r= −0.198,p= 0.041) and miR-377 (r= −0.201,p= 0.041). There was an inverse correlations between dialysate-to-plasma creatinine concentration at 4 hours and PDE expression of miR-192 (r= −0.199,p= 0.040); while mass transfer area coefficient of creatinine correlated with PDE expression of miR-192 (r= −0.191,p= 0.049) and miR-377 (r= 0.201,p= 0.041). Amongst 7 randomly selected patients who had repeat PET after one year, there was a significant correlation between baseline PDE expression of miR-377 and change in ultrafiltration volume (r= −0.852,p= 0.015).Conclusion:The miRNA expression in PDE, including miR-15a, miR-17, miR-21, miR-30, miR-192, and miR-377, correlated with peritoneal transport characteristics. Our result suggests that miRNA may play a role in the regulation of peritoneal membrane function.
Highlights
Peritoneal dialysis (PD) is the first-line treatment of end stage kidney disease in Hong Kong [1]
Amongst all miRNA targets that we examined, peritoneal dialysis effluent (PDE) expression of none of them correlated with the change in Dialysate-to-plasma ratios of creatinine (D/P) in 4 hour or Mass transfer area coefficients of creatinine (MTAC) creatinine over 12 months
In the study we found that the miR-15a, miR-17, miR-21, and miR-192 expression in PDE was substantially higher in prevalent patients than new peritoneal dialysis (PD) patients, while PDE expression of miR17, miR-192 and miR-377 inversely correlated with the peritoneal transport characteristics
Summary
Peritoneal dialysis (PD) is the first-line treatment of end stage kidney disease in Hong Kong [1]. Peritoneal mesothelial cell (PMC) is important in the homeostasis of the peritoneal membrane, and plays active roles in the synthesis and remodeling of extra-cellular matrix [3]. There were modest inverse correlations between ultrafiltration volume and PDE expression of miR-17 (r = −0.198, p = 0.041) and miR-377 (r = −0.201, p = 0.041). Amongst 7 randomly selected patients who had repeat PET after one year, there was a significant correlation between baseline PDE expression of miR-377 and change in ultrafiltration volume (r = −0.852, p = 0.015). Conclusion: The miRNA expression in PDE, including miR-15a, miR-17, miR-21, miR-30, miR-192, and miR-377, correlated with peritoneal transport characteristics. Our result suggests that miRNA may play a role in the regulation of peritoneal membrane function
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