Abstract
That cerebrosides may exist in small amounts in blood has been shown by the development and application of a titrimetric microprocedure for this group of lipids.1, 2, 3 This method, slightly modified, has been employed on lipid extracts of blood, stroma and brain. The data indicate that in erythrocytes, stroma, and brain, much of the lipid which formerly was designated as neutral fat∗ is cerebroside, whereas in plasma only negligible amounts of cerebroside are present. Furthermore, there are indications that the cerebrosides may comprise a significant fraction of the blood lipids in certain pathological conditions.Method. Procedure for hydrolysis: The procedure of Kirk2 was modified to facilitate hydrolysis of the cerebrosides and filtration of the hydrolysate. Aliquot samples of the lipid extract and blanks for initial and total reduction values2 are measured into 10 cc glass-stoppered graduated cylinders. The solvents are evaporated carefully in a boiling water bath. To each of the samples for initial r...
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