Relating nucleic acid and protein indices to growth in Mysis relicta: ration, cycling temperature, and metabolism

Abstract

We investigated growth rate, nucleic acid (DNA, RNA) and protein indices and respira- tion in juvenile (8.5 to 12 mm total body length, 7 to 20 mg wet wt) and young adult (12 to 14 mm, 20 to 30 mg wet wt) Mysis relicta, as a function of temperature, body mass and molt stage in order to develop methods to assess condition or growth in the field. Mysids were exposed to either a preferred temperature (6.5°C) and 3 ration levels, or a range of constant and dielly-cycling (DC) temperatures with ad libitum feeding. Mysid growth parameters (specific rates of growth (SGR), respiration (M O2), and RNA content cell -1 ) integrated the DC temperature experienced as averaged responses weighted by the time spent at each temperature. M O2 peaked at 12.7°C on acute temperature exposure from 4.2°C. MO2 compensation with prolonged temperature exposure occurred at mean diel temperatures ≤8.5°C. Mysids could not survive at 16°C even for 5 h d -1 . These results confirm behavioral observa- tions of temperature preferences. RNA concentration in M. relicta increased with ration and decreas- ing temperatures. Protein:DNA ratio, %protein and SGR increased with ration and then plateaued. Protein:DNA ratio, %protein and DNA:weight ratio did not change with temperature with unlimited feeding. Forward, stepwise, multiple regression models for each experiment and the combined data accounted for 31 to 72% of variability in SGR. Our experimental data provide guidance, a prelimi- nary temperature-correction factor for RNA, and benchmarks for use of nucleic acid and protein indices in assessing growth or condition of M. relicta in the field.