Abstract
Purpose : To measure rejoining of radiation-induced doublestranded DNA-fragments of different sizes and to evaluate the effects of size-resolution in the analysis of rejoining. Material and methods : Normal human fibroblasts (GM5758) were irradiated with photons or accelerated nitrogen ions (linear energy transfer, LET = 125keV μ m -1) and incubated for repair for 0-22h. Double-stranded DNA-fragments from the irradiated cells were separated by pulsed-field gel electrophoresis in the range ~ 5kbp to 5.7 Mbp. Results : For cells irradiated with high LET nitrogen ions, there was an increase in the fast half-time from ~ 5min for fragments < 400kbp to 10 min when all fragments < 5.7 Mbp were measured. Further, the fraction of fragments rejoined by the slowrejoining phase increased significantly for increased threshold sizes. The fraction of unrejoined fragments after 22h and the half-time for the slow-rejoining phase remained constant for all threshold sizes. For cells irradiated with lower doses of low LET radiation the rejoining was shifted towards a slower kinetics when fragments up to 10 Mbp were excluded in the analysis. Conclusion : DNA exclusion-size and resolution may affect the estimates of DNA double-strand break rejoining. Using a lowresolution technique that does not detect small fragments will result in an underestimation, or even disappearance of the fast-rejoining phase. This is due to substantial rejoining of fragments taking place before the fragments are of sufficient size to be monitored.
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