Abstract
SUMMARY In vitro “rejection” of heart perfused by allogeneic sera was studied. Following ligation of pulmonary veins and both venae cavae, the perfusing fluid entered the heart through the aorta, crossed the coronary circulation, and left the heart through the pulmonary artery. The heart action was recorded by means of an electrogram. Rabbit hearts, perfused by rabbit transplantation sera containing corresponding alloantibodies, stopped beating within 7 to 35 min. During the perfusion a great proportion, up to 90%, of antibodies were bound by the heart, as estimated by titration of the perfusing serum by means of mixed agglutination with cell cultures. In addition to antibody, complement played a role in this damage to the heart as evidenced by the inactivity of sera heated for 30 min at 56 C. Rat hearts were also “rejected” by alloantibody-containing transplantation sera, but addition of guinea pig complement to the perfusing rat serum was needed to obtain clear-cut results. Rabbit and rat hearts perfused by normal allogeneic sera showed good function for several hours. These experiments showed that secondary factors such as deposition of polymorphonuclear leukocytes, thrombocytes, or fibrin are not essential for antibody-mediated organ rejection.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
