Regulatory mechanism of Runt related transcription factor 2 in fracture healing by salidrosid

Abstract

Objective To compare and evaluate the differences of curative effects and biological mechanisms underlying the posttraumatic bone healing, as well as the study of the regulation of Runt related transcription factor 2 (Runx2) signal pathway mechanism of osteogenesis by using Salidroside. Methods To establish an close osteotomy model of SD rats. 40 rats divide into experimental group and control group. Using microCT to evaluate and compare the curative efficacy about the posttraumatic bone healing. Using the method of enzyme linked immunosorbent assay (ELISA), the serum concentrations of alkaline phosphatase (ALP), osteoprotegerin (OPG) were assessed. Using the method of real-time quantitative polymerase chain reaction (Real-time PCR), the mRNA expression levels of Runx2, hypoxia inducible factor (HIF) 2A, Wnt4, Wnt10b, β-catenin, glycogen synthase kinase (GSK)-3β, T-cell factor (TCF)-1 in callus tissue were determined. Results At 2 and 4 weeks after surgery, the callus bone structural parameters of microCT were significantly improved in experimental group compared to that of control group (P<0.05). In addition, at weeks 2 and 4 after surgery, the mRNA expressions of Runx2 (2 w: 1.83±0.11, 4 w: 2.23±0.11), HIF2A (2 w: 1.37±0.12, 4 w: 1.50±0.12), Wnt4 (2 w: 1.78±0.10, 4 w: 1.66±0.11), Wnt10b (2 w: 1.89±0.12, 4 w: 1.92±0.13), β-catenin (2 w: 1.65±0.08, 4 w: 1.72±0.10) were significantly increased, whereas the mRNA expressions of GSK-3β (2 w: 0.61±0.08, 4 w: 0.56±0.09) were significantly decreased (P<0.05). The serum concentrations of ALP [2 w: (335.34±9.23) IU/L, 4 w: (312.45±10.56) IU/L]P, OPG [2 w: (40.02±2.90) IU/L, 4 w: (45.56±3.20) pg/ml] were significantly increased (P<0.05). Conclusion Compared with the Control group, the Salidroside group make an effect to fracture healing. Key words: Salidroside; Runt related transcription factor 2 signal pathway; Osteogenesis regulation