Abstract
BackgroundPogostemon cablin is a traditional Chinese medicine (TCM) that is frequently used to treat various gastrointestinal diseases. Patchouli alcohol (PA), a compound extracted from the Pogostemon cablin, has been shown to have anti-tumor efficacy in human colorectal cancer. However, the mechanism of PA’s anticancer effect on gastric cancer (GC) remains unknown.MethodsWe used the public database to obtain the potential targets of PA and genes related to GC. Bioinformatic analyses, such as the Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene Ontology (GO), and protein-protein interactions (PPI), were used for analyzing the potential signal pathways and targets. Cell experiments were also conducted to further explain the impact and molecular mechanism of PA on GC, as well as to confirm the findings of network pharmacology.ResultsUsing network pharmacological analysis, 161 possible targets were identified for the treatment of GC. Network analysis and functional enrichment analysis show that PA produced a marked effect in the treatment of GC through multi-targets and multi-pathways, especially the MAPK and PI3K/AKT signal pathways. In addition, PA showed the inhibition of GC cell proliferation, migration and invasion in cell experiments. According to our findings, PA could also cause G0/G1 phase arrest and apoptosis in GC cells.ConclusionUsing network pharmacology, we aim to uncover the possible molecular mechanism of PA on GC treatment in this research. Cell experiments were also conducted to confirm the therapeutic effect of PA on GC.
Highlights
Gastric cancer (GC) is the world’s fifth most common cancer and the third leading cause of deaths related to cancer [1]
The Patchouli alcohol (PA) treatment group’s cell inhibition rate was significantly higher than the control group (p
The findings indicated that PA significantly inhibited the GC cell proliferation, and induced migration, apoptosis, and invasive activity in a dose-dependent manner
Summary
Gastric cancer (GC) is the world’s fifth most common cancer and the third leading cause of deaths related to cancer [1]. Patchouli alcohol (PA) is a tricyclic sesquiterpene isolated from the Pogostemon cablin plant that has a variety of therapeutic activities such as anti-inflammation [5], anti-gastro ulcerogenic [6], antimicrobial [7], protection against the intestinal epithelial cells fluidity [8], and so on. It is reported that PA has protective effects on oxidative stress, apoptosis, and inflammation of human gastric epithelial cells induced by Helicobacter pylori [9]. Pogostemon cablin is a traditional Chinese medicine (TCM) that is frequently used to treat various gastrointestinal diseases. Patchouli alcohol (PA), a compound extracted from the Pogostemon cablin, has been shown to have anti-tumor efficacy in human colorectal cancer. The mechanism of PA’s anticancer effect on gastric cancer (GC) remains unknown
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