Abstract
An active factor enhancing antibody formation by lymph node cells at the peak of the secondary immune response has been isolated from supernatants of cultured bone marrow taken from non-immune donors. The factor is produced by cells contained in light fractions after separation in a BSA density gradient. It is heat stable and has a molecular weight of 13,000 daltons. The stimulating effect of the factor is manifested in vivo as well as in vitro, thus suggesting that it plays a role in the regulation of antibody synthesis.
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