Abstract
Objective To investigate the effect and mechanism of regulatory dendritic cell (rDC) in allograft rejection.Methods Graft recipients were infused (i.v.) with 1 × 106 rDC,imature dendritic cell (imDC),MatDC or PBS respectively 7 days preceding heterotopic fully MHC-mismatched heart transplantation.Graft survival was monitored by transabdominal palpation of the donor heart.The forkhead box P3 (Foxp3) mRNA expression was determined in lymph node cells at day 7 after the i.v.infusion by reverse transcriptase-polymerase chain reaction (RT-PCR).The sample of allograft had been studied by hematoxylin and eosin (HE) stain and scored according to the International society of Heart.Results rDC prolonged the mean survival time (MST) of allograft,which was 18.6 days,as compared to PBS group (7.1 days; P＜0.01),imDCgroup (12.1 days; P＜0.05) and matDC group (6.8 days; P＜0.01),respectively.Infusion of rDC and imDC resulted in increased foxp3 mRNA expression as compared to infusion of matDC and PBS,respectively.Conclusion Pretreatment with donor type rDC resulted in prolonged survival of a completely MHC-mismatched heart allografl.rDC is more efficacious than the classical imDC in the regulation of the alloimmune response. Key words: Regulatory dendritic cell; Organ transplantation; Allograft rejection
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