Regulation of Tumor Necrosis Factor-related Apoptosis-inducing Ligand Expression in Primary Acute Leukemic Cells by Chemotherapeutics

Shengmei Chen,Zhongxing Jiang,Yanfang Liu,Qiutang Zhang,Hui Sun,Tao Li,Dingming Wan,Jie Ma,Ling Sun

doi:10.4274/tjh.2013.0027

Abstract

Objective: The expression of the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) protein and its regulation by chemotherapeutics were analyzed in primary acute leukemic cells. Materials and Methods: Peripheral blood was collected from 16 patients with acute leukemia on days 0, 1, 3, and 5 of chemotherapy. The mononuclear cells were separated from the peripheral blood, and TRAIL expression was assessed by flow cytometry. The bone marrow mononuclear cells of patients with acute leukemia were separated before chemotherapy and cultured in vitro with VP-16 and/or interferon (IFN). The TRAIL expression level was detected after the cell culture. Results: TRAIL expression in the mononuclear cells of peripheral blood was significantly upregulated on day 1 (p<0.05) and then significantly decreased on day 5 after chemotherapy (p<0.05). Results from the in vitro culture revealed that VP-16 upregulated TRAIL expression in the bone marrow mononuclear cells of patients with acute leukemia, but the binding of VP-16 to IFN did not enhance TRAIL expression as compared with VP-16 alone (p>0.05). onclusion: OA single chemotherapy mechanism for leukemia may suffice to induce TRAIL expression and promote the apoptosis of leukemic cells.Conflict of interest:None declared.

Highlights

The process of programmed cell death or apoptosis has a key effect on the occurrence, development, and stability of an organism
Results from the in vitro culture revealed that VP-16 upregulated tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) expression in the bone marrow mononuclear cells of patients with acute leukemia, but the binding of VP-16 to IFN did not enhance TRAIL expression as compared with VP-16 alone (p>0.05)
We investigated the significance of TRAIL expression in chemotherapy for acute leukemia

Summary

Introduction

The process of programmed cell death or apoptosis has a key effect on the occurrence, development, and stability of an organism. This process has an important role in the inhibition of tumor cell growth and immune surveillance. TRAIL induces apoptosis in tumor cells but has low sensitivity to normal cells [3]. TRAIL can induce the apoptosis of malignant leukemia cells without harming normal cells [5]. The effects of chemotherapeutics on TRAIL expression in primary acute leukemic cells are rarely reported. Our study used flow cytometry to detect TRAIL expression after incubating acute leukemic cells with chemotherapeutics in vivo and in vitro. Our study provides a scientific basis for the clinical application of TRAIL

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Conclusion