Abstract
A system in which the pattern of RNA metabolism has been analyzed in cells destined to lysogeny following phage infection has been employed to test whether the immunity substance is synthesized in the absence of protein synthesis, using repression of mRNA synthesis as a direct indication of the activity of the immunity substance. When protein synthesis is inhibited with chloramphenicol or puromycin, phage mRNA synthesis continues at a constant rate for at least 30 minutes even in cells led to lysogeny. It was also shown that immunity substance is rather stable metabolically.
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