Abstract
A thermosensitive mutant (PD11ts) of Chlamydomonas reinhardi producing the neutral phosphatase at 25°C but not at 35°C was investigated to understand the role of the corresponding gene in the formation of the neutral phosphatase. 1. The mutation only affects the formation of the neutral phosphatase and not that of the alkaline phosphatases. 2. It is recessive to its wild allele. 3. The production of the neutral phosphatase in Pi-starved cells is dependent upon protein synthesis as suggested by the fact that it rapidly stops when the cells are exposed to cycloheximide. 4. As shown by immunodiffusion experiments, PD11tscells grown at 35°C do not contain the antigen related to the neutral phosphatase which is present in cells grown at 25°C.
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