Abstract
Pseudomonas putida wild-type strain P23XI degrades 4-hydroxyphenylacetic acid by an inducible meta -cleavage pathway. Induction experiments with three mutant strains blocked at different reactions in the pathway indicate that the enzymes are synthesized follwing two sequential inductive events. 4-Hydroxyphenylacetic acid induces the 4-hydroxyphenylacetic acid-3-hydroxylase. 3,4-Dihydroxyphenylacetic acid induces at least the three subsequent enzymes (3,4-dihydroxyphenylacetic acid 2,3-dioxygenase, 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase, and 5-carboxymethyl-2-hydroxymuconic acid decarboxylase). Thus, the genes encoding the first four enzymes of this meta -cleavage pathway appear to exist as two operons.
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