Abstract
Accumulation of tetracycline (Tc) into Bacillus subtilis was studied by two methods, one involving a fluorescence assay and the other an absolute determination of accumulated drug. B. subtilis GSY908 harboring B. subtilis plasmid pNS1981 and the plasmidless host strain accumulated equivalent amounts of Tc. Prior exposure of the plasmid-harboring cells to subinhibitory concentration of Tc resulted in marked decrease in accumulation of the drug, indicating that pNS1981-determined Tc resistance is inducible. Of interest was the fact that the amount of Tc accumulated by the Tc-induced plasmid-harboring cells is increased somewhat by the addition of carboxylcyanide-m-chlorophenyl hydrazone (CCCP), an uncoupling reagent. This seems to show that energy-dependent accelerated Tc efflux is involved in decreased Tc accumulation.
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