Abstract
The canonical Wnt pathway inhibits adipogenesis through activation of the transcription factor, TCF/LEF1. Soy phytoestogens, such as genistein (GEN) and daidzein (DAID), are paradoxically shown to both induce and repress adipogenesis. To determine if the concentration of soy phytoestrogen effects adipogenic regulation, 3T3‐L1 fibroblasts were transfected with either a estrogen receptor (ER) or TCF/LEF lentiviral reporter construct. Transfected cells were then treated with GEN or DAID at 100 pM, nM, or uM concentration. Our data showed that both ER and TCF/LEF promoter activity was 2–3 fold greater in cells treated with 100 uM of GEN or DAID (vs. vehicle). In contrast, fibroblasts treated with the pM or nM concentrations exhibited limited promoter activity. Collectively, these data indicate that the anti‐adipogenic properties of soy phytoestrogen may be attributed to high pharmacological doses.
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