Abstract
Luteinizing hormone (lutropin) stimulates glucose-6-phosphate dehydrogenase (EC 1.1.1.49) in isolated nuclei obtained from cow corpus luteum tissue. This increases the biosynthesis de novo of purine and pyrimidine nucleotides and their incorporation into RNA. RNA synthesis was measured after nuclei were incubated for 10 min in buffer media. Several reduction cleavage products and tryptic digests of lutropin inhibited the stimulatory effect of lutropin on RNA synthesis. A site of this inhibition was glucose-6-dehydrogenase.
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