Abstract
Objective To investigate microRNA (miRNA, miR)-221 expression in prostate cancer cells and its influence on prostate cancer cell invasiveness. Methods miR-221 expression levels in prostate cancer cell lines were measured by real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR). The miR-221 overexpression and silencing cell lines were constructed by cell transfection. Effects of the depletion on cell invasiveness were assessed in vitro with Transwell. Results RT-qPCR showed miR-221 was down-regulated in PC3, LNCaP and DU145 cells than in PrEC (F=235.852, P=0.000), in which pairwise comparison also had significant differences. Cell invasiveness assay showed that migration of LNCaP (P=0.000) and DU145 (P=0.000) cells overexpressing miR-221 was significantly slower than in the control group. Conclusion The negative regulation of suppressor of cytokine signaling 1 (SOCS1) by miR-221 can inhibit the invasiveness of prostate cancer cells. Key words: Prostate cancer; MicroRNA-221; Invasiveness; Suppressor of cytokine signaling 1
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
