Regulation of phosphate uptake kinetics in the bloom-forming dinoflagellates prorocentrum donghaiense with emphasis on two-stage dynamic process

Abstract

Phosphorus is an essential element for the growth and reproduction of algae. In recent years, the frequent outbreaks of algal blooms caused by eutrophication have drawn much attention to the influence of phosphate (P) uptake on the growth of algal cells. The previous study only considered the effect of total P pools on the P uptake process of algal cells and considered the process as one stage, which is insufficient. P uptake by algae is actually a two-stage kinetic process because in many algae species, surface-adsorbed P pools account for a large proportion of total P pools. In this paper, we fit one-stage and two-stage models of P uptake by algae to our experimental data on short-term uptake kinetics of algae Prorocentrum donghaiense under P-deplete and P-replete conditions at 24°C. According to the experimental results, P. donghaiense possesses different P uptake characteristics under different P concentrations. P. donghaiense grows faster and exponentially for longer periods of time under P-replete condition. Ranges of change of Qc (cell quota of intracellular P) and Sp (cell quota of surface-adsorbed P) during the culture time are obviously larger under P-replete condition than those under P-deplete condition. The value of Kp (represents the impact of P-starvation on P uptake rate) in one-stage model under P-deplete condition is smaller than that under P-replete condition, which is opposite to results of two-stage model and does not meet the actual biological significance of Kp. The two-stage model gives more reasonable and realistic explanations to the process of P uptake by algae no matter from the perspective of intuitive fitting effect, biological significance of parameters, statistical test results or essential dynamic process. These results, combined with long-term lab and field data in ocean, could be used to effectively predict algal blooms.