Abstract
Lower available P (aP) was used as a base value in nutritional strategies for mitigating P pollution by animal excreta. We hypothesized that the mechanism regulating phosphate transport under low dietary P might be related with the AMPK signal pathway. A total of 144 one-day-old Arbor Acres Plus broilers were randomly allocated to control (HP) or trial (LP) diets, containing 0.45 and 0.23% aP, respectively. Growth performance, blood, intestinal, and renal samples were tested in 21-day-old broilers. Results shown that LP decreased body weight gain and feed intake. Higher serum Ca and fructose, but lower serum P and insulin were detected in LP-fed broilers. NaPi-IIb mRNA expression in intestine and NaPi-IIa mRNA expression in kidney were higher in the LP group. AMP: ATP, p-AMPK: total AMPK, and p-ACC: total ACC ratios in the duodenal mucosa were decreased in the LP group, whereas the p-mTOR: total mTOR ratio increased. These findings suggested that the increase in phosphate transport owing to LP diet might be regulated either directly by higher mTOR activity or indirectly by the suppressive AMPK signal, with corresponding changes in blood insulin and fructose content. A novel viewpoint on the regulatory mechanism underlying phosphate transport under low dietary P conditions was revealed, which might provide theoretical guidelines for reducing P pollution by means of nutritional regulation.
Highlights
Phosphorus (P) is an essential nutrient for skeletal development in rapidly growing bird
These findings suggested that the increase in phosphate transport owing to LP diet might be regulated either directly by higher mTOR activity or indirectly by the suppressive AMPK signal, with corresponding changes in blood insulin and fructose content
We examined the effects of reduced dietary available P (aP) on blood parameters, phosphate transport mRNA expression in intestine and kidney, and the AMPK signal pathway protein expression in growing broilers
Summary
Phosphorus (P) is an essential nutrient for skeletal development in rapidly growing bird. The efficiency of P utilization has been focused on in contemporary research. To address this issue, poultry nutritionists have developed several nutritional strategies, including the estimation of precise P requirements [2], dietary supplementation with feed additives, such as microbial phytase [3], vitamin D3 metabolites [4], and organic acids [5], as well as feed ingredients with low phytin-P [6, 7]. Most of the effective nutritional strategies set at lowering dietary available P (aP). Providing lower amounts of P than the estimated requirement was an effective method of reducing the excretion of P, and it was independent of phytase supplementation [8]. Another study showed that higher adaptive capacity was found in modern broilers subjected to early dietary P restrictions [9]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
