Regulation of Pancreatic Exocrine Function In Vitro: Initial Steps in the Actions of Secretagogues

Abstract

The sequence of morphological and biochemical events in the pancreas during the synthesis and secretion of pancreatic enzymes is reasonably well-established (23); however, only during the past 5 years have we begun to elucidate the mechanisms through which pancreatic secretagogues regu­ late the secretory process. This recent progress has resulted primarily from the development of in vitro preparations such as slices of pancreas, mi­ crodissected acini, and dispersed acinar cells. Studies using these prepara­ tions coupled with the application of electrophysiologic techniques have provided insight into the biochemical and electrical changes that constitute the initial steps in the action of secretagogues on pancreatic enzyme secre­ tion. The present paper reviews the major findings that have helped to elucidate the initial sequence of changes effected by secretagogues in various in vitro preparations of pancreas.