Abstract
The redox-mediated regulation of palmitoylcarnitine oxidation was studied in isolated rat liver mitochondria in which the mitochondrial free NADH/NAD + ratio was controlled by graded concentrations of acetoacetate and ketomalonate in a rotenone and malonate-inhibited system in the presence of ADP. The NADH/ NAD + ratio was buffered kinetically by adjusting the concentrations of the hydrogen acceptor substances and determined by calibrated NAD(P)H fluorometry of the mitochondrial suspension. A two-fold variation in the β-oxidation rate and a five-fold variation in the free NADH/NAD + ratio was obtained in the presence of rotenone. A non-linear negative correlation was found between the acetyl-CoA concentration and the β-oxidation rate and a negative correlation between the long-chain acyl-CoA concentration and the β-oxidation rate. The data indicate that the redox state is a partial controller of the β-oxidation rate in liver mitochondria. The contribution of acetyl-CoA, a putative regulator of β-oxidation at the acyl-CoA thiolase step is small under the conditions used.
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