Regulation of p53 mediated transactivation by the β-subunit of protein kinase CK2

Abstract

The growth suppressor protein p53 plays a main part in cellular growth control. Two of its key functions are sequence specific DNA binding and transactivation. Functions of p53 in growth control are regulated at least in part by its interaction with protein kinases. p53 binds to protein kinase CK2, formerly known as casein kinase 2, and it is phosphorylated by this enzyme. CK2 is composed of two regulating β-subunits and two catalytic α- or α′-subunits and the interaction with p53 is mediated by the regulatory β-subunit of CK2. Recently we showed that the β-subunit could inhibit the sequence specific DNA binding activity of p53 in vitro. Based on this finding, we asked if a coexpression of the β-subunit of CK2 with p53 in mammalian cells could inhibit the DNA binding activity of p53 in a physiological context. We found that the coexpression of the β-subunit showed the same inhibitory effect as in the previous assays with purified proteins. Then, we investigated the effects of the coexpression of the β-subunit of CK2 on the transactivation and transrepression activity of p53. We found that transactivation of the mdm2, p21 WAF1/CIP1 and cyclin G promoter was inhibited in three different cell lines whereas transactivation of the bax promoter was not affected in COS1 cells but down-regulated in MCO1 and SaosS138V21 cells. p53 mediated transrepression of the fos promoter was not influenced by coexpression of the CK2 β-subunit. Taken together we propose a cell type dependent fine regulation of the p53 transactivation function by the CK2 β-subunit in vivo, which does not affect p53 mediated transrepression.