Abstract
Using quantitative competitive reverse transcription-polymerase chain reaction (RT-PCR), we found an increased level of Na + channel β1 (Na β1) mRNAs in spinal cord astrocytes and in the B50 neuroblastoma cell line after exposure to 1 mM dibutyryl cAMP. In contrast, the calcium ionophore (1 μM A23187) did not affect Na β1 mRNA levels in these cells. Further, we amplified full length coding region of Na + channel β2 (Na β2) mRNA from rat optic nerve and cultured astrocytes using RT-PCR. It appeared that the Na β2 mRNA level was increased by dibutyryl cAMP, but not by A23187, in spinal cord astrocytes. These findings suggest that the Na β1 and Na β2 mRNA levels in spinal cord astrocytes are influenced by increased cAMP but not by calcium.
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