Regulation of mouse hepatitis virus RNA synthesis by heterogeneous nuclear ribonucleoprotein A1.

Abstract

Mouse hepatitis virus (MHV) RNA synthesis, including replication of viral genome and transcription of subgenomic mRNAs, has been shown to be regulated by several viral RNA elements, including 5’-untranslated region (5’-UTR), cis- and trans-acting leader RNA, intergenic (IG) sequence, and 3’-UTR (Lai and Cavanagh 1997). Biochemical evidence suggests that these regulatory sequences likely interact with each other either directly or indirectly, probably through protein-RNA and protein-protein interactions involving both viral and cellular proteins (Zhang and Lai 1995). Several cellular proteins have been found to bind these regulatory sequences by UV-crosslinking experiments (Furuya and Lai 1993, Huang and Lai 1999); three of these proteins have been identified to be heterogeneous nuclear ribonucleoprotein Al (hnRNP A1) (Li et al 1997), polypyrimidine-tractbinding protein (PTB) (Huang and Lai 1999, Li et al 1999), and poly(A)-binding protein (PABP) (Spagnolo and Hogue 2000).KeywordsHeterogeneous Nuclear RibonucleoproteinMouse Hepatitis VirusHoward Hughes Medical InstitutehnRNP ProteinSubgenomic mRNAThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.