Abstract

Embryonic stem cells (ESC) have the capacity to differentiate into all cell types of the organism (pluripotency). Regulation of self-renewal and differentiation is critical in order to maintain this property. The possibility that some key ESC fate determinants are not identified yet prompted us to create a collection of ESC clones that carry nested chromosomal deletions. We used this library of more than 1000 clones to screen for embryoid body (EB) formation determinants, followed by complementation experiments of the phenotypic groups in order to identify new genomic elements regulating ESC fate.

Full Text
Paper version not known

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.