Abstract
Zinc oxide (ZnO) nanoparticles (NPs) have been applied in numerous industrial products and personal care products like sunscreens and cosmetics. The released ZnO NPs from consumer and household products into the environment might pose potential health issues for animals and humans. In this study the expression of microRNAs and the correlations of microRNAs and their targeted genes in ZnO NPs treated chicken ovarian granulosa cells were investigated. ZnSO4 was used as the sole Zn2+ provider to differentiate the effects of NPs from Zn2+. It was found that ZnO-NP-5 μg/ml specifically regulated the expression of microRNAs involved in embryonic development although ZnO-NP-5 μg/ml and ZnSO4-10 μg/ml treatments produced the same intracellular Zn concentrations and resulted in similar cell growth inhibition. And ZnO-NP-5 μg/ml also specifically regulated the correlations of microRNAs and their targeted genes. This is the first investigation that intact NPs in ZnO-NP-5 μg/ml treatment specifically regulated the expression of microRNAs, and the correlations of microRNAs and their targeted genes compared to that by Zn2+. This expands our knowledge for biological effects of ZnO NPs and at the same time it raises the health concerns that ZnO NPs might adversely affect our biological systems, even the reproductive systems through regulation of specific signaling pathways.
Highlights
Tons of Zinc Oxide (ZnO) nanoparticles (NPs) are produced annually in the world [1]
ZnO NPs have been reported to result in adverse effect on organisms and to change the expression of genes related to cytoskeletal transport, cellular respiration, and reproduction in Daphnia magna [16], and in our recent study we found ZnO NPs regulate the protein expression too [41]
As reported in our previous publication [41], the color, density, size and surface area of spherical ZnO NPs used in our studies were milk white, 30nm, 50m2/g and 5.606g/cm3
Summary
Tons of Zinc Oxide (ZnO) nanoparticles (NPs) are produced annually in the world [1]. ZnO NPs are widely used in medical, industrial, and household applications [1,2,3,4,5,6,7,8], as anti-virus and anti-bacteria [9,10,11] agents due to their small size and excellent biological properties. Zinc Oxide Nanoparticles Regulate microRNA the release of ZnO NPs from consumer and household products into the environment is much possible and they may pose potential threat to the animals and humans [12,13,14,15,16]. Uptake of ZnO NPs by the gastrointestinal tract is considered as one of the very important routes [24]
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