Abstract
Microcystin-LR is a cyclic heptapeptide hepatotoxin produced by harmful cyanobacteria. A panel of microRNAs containing miR-451a were found to be significantly changed in normal human liver cells HL7702 after exposure to microcystin-LR (MC-LR) in our previous study. However, the functions of miR-451a in hepatotoxicity induced by MC-LR remained unclear. The study aimed to investigate the impacts of miR-451a in HL7702 cells following treatment with 5 or 10 μM MC-LR. The comet assay indicated that MC-LR can influence Olive tail moment (OTM) in HL7702 cells. Furthermore, increase of miR-451a significantly repressed DNA damage and the protein expression level of γ-H2AX induced by MC-LR. Moreover, over-expression of miR-451a inhibited the expression level of p-AKT1 protein in cells following treatment by MC-LR. These results showed that miR-451a may protect from MC-LR-induced DNA damage by down-regulating the expression of p-AKT1, which provides new clues for the diagnosis and therapy policies for liver damage induced by MC-LR.
Highlights
Microcystins (MCs) are a series of cyclic heptapeptide toxins generated by cyanobacteria that pose serious threats to the ecological system and public health
AKT is in a numerous serine and threonine protein kinases that plays an important role in protein cell of miR-451a papillary of thyroid carcinoma significantly reduced the AKT1 and p-AKT1 proliferation, cell migration and DNA damage
This study showed there was a vital linkage among MC-LR, miR-451a and AKT1 in HL7702 showed was a probably vital linkage
Summary
Microcystins (MCs) are a series of cyclic heptapeptide toxins generated by cyanobacteria that pose serious threats to the ecological system and public health. Showed that MC-LR can induce DNA double strand breakage and oxidant-induced DNA damage in MicroRNAs (miRNAs) are a family of non-coding RNAs with 18 to 24 nucleotides. It can vivo and in vitro [10,11]. MiRNA play a significant roles (miRNAs) are a family of non-coding RNAs with 18 to 24 nucleotides It can in numerous cellular processes, including differentiation, proliferation, apoptosis and metabolism negatively regulate gene expression or suppress translation and stability of mRNA via pair combined and development cancers [14]. In hepatic cells were investigated in this study, which provides new clues for the diagnosis and treatment policies for liver damage induced by MC-LR
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