Regulation of long noncoding RNA by RCK/p54 (LB205)

Abstract

RCK/p54, or DDX6, is a member of the conserved DEAD‐box RNA helicase family, and is encoded by the DDX6 gene in human. Early studies of Dhh1, the yeast homolog of RCK/p54, have shown that that Dhh1 is a core component of cellular decapping machinery and a general translational repressor. In human cells, it has been shown that that RCK/p54 is essential for both miRNA‐mediated and AU‐Rich‐Element‐dependent translational repression. In addition, depletion of RCK/p54 disrupts the formation of P‐bodies, the putative foci of mRNA decay and storage. These results suggest that RCK/p54 is required for translational repression and mRNA turnover. Studies in C.elegans, Drosophila, and Xenopus have also shown that DDX6 homologs function in storage of maternal messages during early development. These findings implicate that RCK/p54 participates in multiple post‐transcriptional gene modulation mechanisms.Long noncoding RNAs (lncRNAs) has recently emerged as essential players in the control of cell fate and the regulation cellular physiology. Extensive studies have been launched to decode the functions of lncRNAs, but fewer studies described the regulation of lncRNAs to date. In this study, we tried to investigate whether lncRNAs, like mRNAs, are subjects to regulation by RCK/p54. Here, we combine RCK/p54 RIP‐seq and RCK/p54‐depletion RNA‐seq for an integrative analysis. Our result provides evidences that RCK/p54 directly interacts with lncRNAs and regulates their function.