Regulation of Lipid metabolism in Bombyx mori using minor millet malt

Abstract

Current study investigates transesterification and regulation of lipid metabolism activity of minor millet malt using Bombyx mori as model organism. Minor millet malt extracts were examined for transesterification reaction on sunflower oil through gas chromatographic analysis. In-vivo lipid regulation was investigated on silkworm larvae and in-vitro biochemical assays were carried out to evaluate lipid levels using spectophotometric techniques. Little millet treated oil showed decreased retention time with 1.69 min and highest retention time was observed in oil treated with kodo millet and foxtail millet with 1.96 min. In the current study, silk worm larvae were fed with mulberry leaves dipped with millet malt extract in order to examine their morphological, body weight and biochemical changes. Silk worm larvae fed with little millet showed overall best results exhibiting lesser increase in the body weight gain by 0.9±0.16gms during the course of treatment. Biochemical examination of little millet treated silkworm larvae showed significantly lower triglyceride levels (24.67±2.3mg/dl), VLDL (4.93±0.4mg/dl) and glucose (74.67mg/dl). Therefore, minor millet malt extracts can be potential agents for further exploration of its lipolytic properties in mammals in order to understand the mechanism of lipid regulating activity by minor millets.