Abstract

Intracellular pH (pH i) affects smooth muscle function, yet little is known concerning its regulation. I have therefore investigated pH regulation in rat uterus, using 31P-NMR spectroscopy. A change in extracellular pH(pH e) of 1 pH unit (7.4 to 6.4) elicited a 0.29 change in pH i; smaller changes in pH o were accompanied by proportionately smaller changes in pH i. The pH changes were reversible. There was no fall of uterine ATP or phosphocreatine during the pH changes.

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