Regulation of inflammatory mediator expression in bovine endometrial cells: effects of lipopolysaccharide, interleukin 1 beta, and tumor necrosis factor alpha.

Yong Qin Koh,Fatema B Almughlliq,Kanchan Vaswani,Murray D Mitchell,Hassendrini N Peiris

doi:10.14814/phy2.13676

Yong Qin Koh, Fatema B Almughlliq + Show 3 more

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https://doi.org/10.14814/phy2.13676

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Journal: Physiological Reports	Publication Date: Apr 30, 2018
Citations: 22	License type: CC BY 4.0

Affiliation: University of Queensland

Abstract

An abnormal uterine environment can influence maternal–fetal communication, conception rate and disrupt normal embryo development, thereby affecting fertility and the reproductive performance of dairy cows. Animal variability means that development of endometrial cell lines with appropriate characteristic are required. We evaluated the effect of an infectious agent (i.e., bacterial lipopolysaccharide; LPS) and proinflammatory mediators (i.e., Interleukin 1 beta; IL‐1β, and tumor necrosis factor alpha; TNFα) on inflammatory mediator gene expression and production by bovine endometrial epithelial (bEEL) and stromal (bCSC) cell lines. Expression of CXCL8/IL8, IL1A, IL1B, and IL6 cytokine genes was significantly upregulated in both epithelial and stromal cells when treated with LPS and IL‐1β. LPS treatment of epithelial cells (compared with treatment by IL‐1β and TNFα) exhibited greater CXCL8/IL8, IL1A, IL1B, and IL6 cytokine gene expression. Whereas, in stromal cells, IL‐1β treatment (compared with LPS and TNFα) exhibited greater CXCL8/IL8, IL1A, IL1B, and IL6 cytokine gene expression. Interestingly, bEEL and bCSC cells treated with IL‐1β increased IL1B gene expression, suggesting that IL‐1β may act unusually in an autocrine‐positive feedback loop. Cytokine production was stimulated by these agents in both cell types. We suggest that the characteristics of these two cell lines make them excellent tools for the study of intrauterine environment.

Highlights

IntroductionBidirectional maternal–fetal communication (i.e., conceptus-endometrial cross-talk) is critical for the establishment, maintenance, and progression of a successful pregnancy (Wolf et al 2003)
Bidirectional maternal–fetal communication is critical for the establishment, maintenance, and progression of a successful pregnancy (Wolf et al 2003)
The RT-PCR array data for inflammatory mediator gene expression in bovine endometrial epithelial cells treated with LPS, IL-1b, and TNFa are presented in Figures 1–3 and in Supplemental Data S1

Summary

Introduction

Bidirectional maternal–fetal communication (i.e., conceptus-endometrial cross-talk) is critical for the establishment, maintenance, and progression of a successful pregnancy (Wolf et al 2003). In cattle embryonic losses that occur in the early days of pregnancy (days 8–17) have been attributed to poor communication between the conceptus and maternal environment (Thatcher et al 2001). Postpartum uterine disease is one of the leading causes of reduced fertility in dairy cattle. Significant bacteria responsible for postpartum uterine disease are Escherichia coli, Trueperella pyogenes, and pathogenic anaerobic bacteria (Sheldon and Owens 2017). Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.

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