Abstract

Simultaneous addition of concanavalin A (Con A) and lipopolysaccharide (LPS) to cultures of rat spleen lymphocytes resulted in a synergistic effect on DNA synthesis as measured by increased [ 3H]thymidine uptake after 3 days. This effect was maximal when 10 μg of LPS was added to understimulating doses of Con A (synergistic index = 14) and diminished with increasing doses of the mitogen. In contrast to increasing concentrations of serum factors, LPS was not able to unblock the nonresponse of lymphocytes stimulated with supraoptimal doses of Con A. LPS did not exert its adjuvant effect by stimulating lymphocytes with the help of soluble factors released by Con A-activated cells. Both Con A and LPS seem rather to act together on a distinct population of T-cells which can be separated on nylon columns and respond twice as much as nonseparated cells to their synergistic combination. Rat B-cells were unresponsive to stimulation with Con A and LPS added alone or simultaneously. These results help to better understand some of the mechanisms involved in the immunological enhancement observed with LPS.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.