Regulation of human gonadotropins. XIV. Gel filtration and electrophoretic analysis of endogenous and extracted immunoreactive human follicle-stimulating hormone of pituitary, serum and urinary origin.

Abstract

To obtain further information regarding the nature of the immunoreactive material measured as FSH with one radioimmunoassay method, a number of different preparations were subjected to electrophoresis (7.5% polyacrylamide gel, boric acid-EDTA-tris buffer, pH 8.9) or gel filtration (Sephadex G-100, 0.05m ammonium bicarbonate buffer, pH 8.0) and resulting elution profiles of immunoreactive material measured by radioimmunoassay. Preparations studied included sera from non-pregnant and pregnant subjects, serum from a patient with choriocarcinoma, urinary extract and pituitary extract. With the exception of activity in sera from pregnant subjects, immunoreactive material was eluted as a single symmetrical peak, nearly coincident with that observed for highly purified, radioiodinated human FSH of pituitary origin. In contrast, no discrete peak of activity was located when sera from women at all stages of pregnancy were examined in a similar fashion. Instead, low amounts of “FSH” activity were identified over a ...