Abstract
Objective To investigate the effects of human ovarian fragmentation and AKT stimulation on follicular in vitro activation. Methods The Hippo signaling pathway related proteins Yes associated protein (YAP), p-YAP, cellular communication network (CCN) growth factor and baculoviral inhibitors of apoptosis repeat containing (BIRC) apoptosis inhibitors were detected by fragmenting human ovarian tissues in vitro to determine the effects of the fragmentation on ovaries. p-AKT expression was detected after adding AKT stimulator to the fragmented ovarian tissues in vitro, and latissimus dorsi muscle transplantation was performed in nude mice to explore whether follicular development could be activated. Results The fragmentation decreased the expression of p-YAP and increased the expression of CCN growth factor and BIRC apoptosis suppressor. The expression of p-AKT was increased in the fragmented ovarian tissue cultured with AKT stimulant in vitro. Two weeks after latissimus dorsi muscle transplantation in nude mice, there were primordial follicles and new blood vessels in the ovarian tissue. Conclusion Human ovarian tissue fragmentation can disrupt Hippo signaling pathway. Treatment with AKT stimulation of the ovary can stimulate follicular development. Key words: Fragmentation; AKT stimulation; In vitro activation; Follicular development; Premature ovarian failure
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