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Abstract
It is observed that the decrease in the energy charge, increase in P i, NH 4 +, and fructose-6-phosphate observed in stimulated frog muscle act synergistically in increasing the activity of rabbit muscle phosphofructokinase 300-fold over its activity observed at the concentrations of above effector substrates found in the muscle at rest. The activity of phosphofructokinase at various concentrations of Mg 2+ and various fixed concentrations of NH 4 +, at levels of energy charge and P i corresponding to the resting and stimulated muscle were also studied. These results suggest that variations in the concentrations of effectors of phosphofructokinase resulting from contraction of muscle are responsible for the increase in the activity of enzyme in stimulated muscle and that this activation may not necessarily be geared to the contractile process itself as postulated by Karpatkin el al.
Published Version
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