Regulation of glucose and lipid metabolism by insulin and glucagon in vivo and in vitro in common carp Cyprinus carpio L.

Intestinal microbiota and lipid metabolism responses in the common carp (Cyprinus carpio L.) following copper exposure

An 82-day study was conducted to assess the effect of the dietary lipid levels on growth performance, feed utilization, lipid deposition, and hepatopancreas lipometabolism of large-sized common carp (Cyprinus carpio). Six isonitrogenous (300 g/kg protein) pelletized diets with different dietary lipid levels (30, 60, 90, 120, 150, and 180 g/kg) were fed in triplicate to fish groups with 75 individuals (with an initial mean weight of 247.00 ± 16.67 g). The results showed that there was a significant increase in weight gain (WG) rate (WGR), specific growth rate (SGR), and protein efficiency ratio (PER) as dietary lipid levels increased from 30 to 60 g/kg (p < 0.05) and then there was a decline. Feed conversion rate (FCR) was observed to be significantly lower in 60 g/kg lipid treatments (p < 0.05). Muscle crude protein contents were obtained to be significantly higher in 60 and 90 g/kg treatments (p < 0.05). The crude lipid content in the hepatopancreas increased significantly with an increase in dietary lipid levels (p < 0.05). The expression of lipoprotein lipase (LPL) and carnitine palmitoyltransferase-1 (CPT1) in the hepatopancreas was significantly downregulated with an increase in dietary lipid levels while the expression of growth hormone (GH), insulin-like growth factor-1 (IGF-1), fatty acid synthase (FAS), acetyl-CoA carboxylase-1 (ACC-1), and sterol regulatory element binding protein (SREBP) was upregulated first in 30 and 60 g/kg lipid treatments and then downregulated significantly in other treatments. The results revealed that excess dietary lipid supplements (more than 60 g/kg) would inhibit WG and would aggravate the lipid decomposition in the hepatopancreas. Based on WGR and FCR, the dietary lipid levels of 59.5 and 70.4 g/kg were optimal for growth performance and feed utilization of large-sized common carp.

SREBP Activity Is Regulated by mTORC1 and Contributes to Akt-Dependent Cell Growth

The Later Effects of DHA in Diet on Regulating Transcription of Lipid Genes of Broiler

High-fat diet is regarded as crucial inducers of oxidative stress, inflammation, and metabolic imbalance. In order to investigate the ameliorative potential of resveratrol against the progression of liver injury towards steatohepatitis, common carp (Cyprinus carpio) were distributed into six experimental groups and were fed with a normal-fat diet, a high-fat diet, and supplemented with resveratrol (0.8, 1.6, 2.4, and 3.2 g/kg diet) for 8 weeks. The high-fat diet decreased the antioxidant capacities, as well as causing the inflammatory response and lipid deposition of common carp. Resveratrol induced a marked elevation in the final body weight, weight gain rate, condition factor and significant decrease in the feed conversion ratio. Moreover, dietary resveratrol showed a significant decrease in the alanine aminotransferase, aspartate aminotransferase, triglyceride and low-density lipoprotein levels, which was accompanied by an increase in high-density lipoprotein concentration in serum. A significant elevation in total superoxide dismutase, catalase, glutathione peroxidase and a decreased malondialdehyde content were observed, along with a substantial elevation in antioxidant activities were found. Additionally, fish fed with resveratrol had an up-regulation of hepatic catalase, copper, zinc superoxide dismutase, glutathione peroxidase 1a, and glutathione peroxidase 1b gene expression via Nrf2 signaling pathway. Expectedly, our results also demonstrated that resveratrol regulates hepatic lipid metabolism in fish by inhibiting the expression of hepatic lipogenesis genes (acetyl-CoA carboxylase 1, fatty acid synthase, and sterol regulatory element binding protein 1), fatty acid uptake-related genes of lipoprotein lipase, and β-oxidation-related genes via PPAR-γ signaling pathway. Furthermore, dietary resveratrol reduced inflammation, as evident by down-regulating the interleukin-1β, interleukin-6, interleukin-8, and tumor necrosis factor-α expression levels and upregulating the interleukin-10 and transforming growth factor-β2 expression levels via NF-κB signaling pathway. As a whole, our results demonstrated that resveratrol defensed the impacts against high-fat diet on the serum biochemical, hepatic antioxidants, inflammation, and lipid metabolism.

Apelin plays pivotal roles in fluid homeostasis, food intake and energy metabolism in mammals. However, the regulatory role of apelin in glucose metabolism in teleosts is still unclear. In this study, we investigated the regulatory role of Pyr-apelin-13 in glucose metabolism of common carp (Cyprinus carpio L.). The results showed that apelin mRNA abundance was significantly increased in the foregut and hepatopancreas after glucose injection. Insulin and glucagon also up-regulated apelin mRNA levels in the foregut, hepatopancreas and hepatocytes. In vivo, intraperitoneal injection of Pyr-apelin-13 induced hypoglycaemia through the up-regulation of mRNA expression and activity of hexokinase (HK) coupled with the down-regulation of mRNA expression and activity of glucose-6-phosphatase (G6Pase) in the hepatopancreas. In vitro, upon incubation of the primary hepatocytes with Pyr-apelin-13 (0, 10, 100 and 1000 nM), the mRNA expression of glucokinase (GK) was increased, whereas the mRNA expression of phosphoenolpyruvate carboxykinase (PEPCK) and G6Pase was reduced. For glucose transport, the glucose transporter 2 (GLUT2) mRNA expression increased in the hepatopancreas, intestine, primary enterocytes and hepatocytes after Pyr-apelin-13 treatment. Collectively, our data may provide a basis for further studies on elucidation of the role and mechanism of apelin in glucose metabolism of teleosts.

This study was aimed to estimate the selectivity properties of multifilament trammel and gillnets for common carp ( Cyprinus carpio L., 1758) in Marmara Lake in Manisa, Turkey. Trammel and gillnets of 4, 6, 8, and 10 cm stretched mesh size and 210 d/2 no twine thickness were tested in the Marmara Lake. In addition, selectivity parameter of 11, 12, 13 and 14 cm mesh size of trammel and gillnets were modelled. SELECT method was used to estimate the selectivity parameters. Experiments were carried out monthly in the three different stations identified eastern, middle and western areas which represent longitudinal length of the lake. A total of 36 trials were conducted both nets. A total of 119 common carps were caught ranges between 11.3 - 49.0 cm total lengths. According to the Bi- normal model, which gave the lowest deviance for both trammel and gillnets, selectivity curves were estimated for 4, 6, 8, 10, 11, 12, 13 and 14 cm stretched mesh size modelled lengths as 12.98, 19.47, 25.96, 32.45, 35.70, 38.94, 42.19 and 45.43 cm for trammel nets, 12.40, 18.60, 24.80, 31.00, 34.10, 37.20, 40.30 and 43.40 cm for gillnets, respectively. When considering minimum landing size (40 cm total length), it is recommended that both nets under 13 cm mesh size should be prohibited for common carp fishery in the Lake Marmara. Keywords: Trammel net, gillnets, common carp Cyprinus carpio, size selectivity, Lake Marmara

This study was conducted to estimate the effect of dietary conjugated linoleic acids (CLA) on intramuscular and subcutaneous fat deposition in Yellow Breed × Simmental cattle. The experiment was conducted for 60 days. The results showed that the average backfat thickness, (testicles + kidney + pelvic) fat percentage and subcutaneous fat percentage in dietary CLA were significantly lower than in the control group, while intramuscular the fat percentage was significantly higher. Compared to the control group, the Longissimus muscle enzyme activities of lipoprotein lipase (LPL), fatty acid synthase (FAS) and acetyl-coenzyme A carboxylase (ACC) in dietary CLA and the subcutaneous fat enzyme activities of LPL, hormone-sensitive lipase (HSL) and carnitine palmitoyltransferase-1 (CPT-1) were significantly increased. Similarly, compared to the control group, the Longissimus muscle sterol regulatory element binding protein 1 (SREBP-1), FAS, stearoyl-coenzyme A desaturase (SCD), ACC, peroxisome proliferator-activated receptor γ (PPARγ), heart fatty-acid binding protein (H-FABP) and LPL gene expression in dietary CLA were significant increased, as were the subcutaneous fat of PPARγ, H-FABP, LPL, CPT-1 and HSL in dietary CLA. These results indicated that dietary CLA increases IMF deposition mainly by the up-regulation of lipogenic gene expression, while decreasing subcutaneous fat deposition mainly by the up-regulation of lipolytic gene expression.

Swimming, grazing and social behaviour of rohu Labeo rohita (Hamilton) and common carp Cyprinus carpio (L.) in tanks under fed and non-fed conditions

This study was conducted to estimate different levels of protein supplementary diet on gene expressions related to intramuscular deposition in early-weaned yaks. Results showed that supplementary dietary protein significantly increased final weight, average daily gain (ADG), intramuscular fat (IMF), serum free fatty acid (FFA), total triglycerides, total cholesterol (Ch), low-density lipoprotein cholesterol (LDL) and high-density lipoprotein cholesterol (HDL) content. There was a quadratic response of ADG, IMF, FFA, Ch, HDL and LDL to dietary crude protein (CP) level. Lipoprotein lipase (LPL), fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC) enzyme activities were significantly increased by supplementary dietary CP, while hormone-sensitive lipase (HSL) and carnitine palmitoyltransferase-1 (CPT-1) activities were significantly decreased. LPL, ACC and FAS enzyme activities showed quadratic increase as dietary CP increased. Peroxisome proliferator-activated receptor γ (PPARγ), LPL, FAS, sterol regulatory element binding protein 1 (SREBP-1), ACC, stearoyl-CoA desaturase (SCD) and heart fatty-acid binding protein (H-FABP) gene expression were significantly increased by supplementary dietary CP, while HSL and CPT-1 gene expression were significantly decreased. PPARγ, LPL, SREBP-1, ACC and H-FABP gene expression showed quadratic increase as dietary CP increased. These results indicated that supplementary dietary protein increased IMF accumulation mainly to increased intramuscular lipogenic gene expression and decreased lipolytic gene expression.

Diel rhythmicity of grazing, swimming, resting andsocial interactions of rohu (Labeorohita)(weight66.5^68.3g) and common carp (Cyprinus carpio)(79.9^82.0g) were observed in1m 2 simulated ponds usingvideoimages. Fishbehaviour was monitored duringafull24-hperiod,startingat08:00hourswitha15-minrecording, whichwas repeated every 3h. Rohu spentmoretimegrazingduringthedaythanatnight.Graz-ingactivity peakedat thebeginningandat the endofthe day. Grazing and resting of rohu were negativelycorrelated.Commoncarpgrazeddayandnight,show-ing no distinctgrazing peak. Considering thegrazingtime of rohu and common carp, a feeding schedulewithtwofeedingtimesat07:30and16:30hoursmightbeappropriateforbetterfoodintakeandconversioninrohu^commoncarppolyculturesystem.Keywords: swimming, grazing, behaviour, rohu,commoncarpIntroductionTogether, rohu Labeo rohita (Hamilton) and commoncarp Cyprinus carpio (L.) contribute 26% (rohu 11%,commoncarp15%)ofthetotalworldfreshwateraqua-culture production outside China (FAO 2005). Nowa-days, rohu^common carp polyculture is being widelypracticed in South Asia. Rohu is a highlyappreciatedconsumption ¢sh, fetching good market prices (Dey,Paraguas, Bhatta, Alam, Weimin, Piumsombun,Koeshandrajana,DungSRahman,Wahab& Verdegem 2007), while common carp is gainingquick popularity as a bottom feeder in South Asiabecause it has a faster growth rate than the nativebottom feeder mrigal Cirrhinus cirrhosus (Bloch)(Milstein,WahabRRahman2006).The eiects of addition of arti¢cial feed and diier-ent densities of common carp on natural food avail-ability, food intake and growth and production inrohu ponds were studied (Rahman, Verdegem,Nagelkerke,Wahab, Milstein & Verreth 2006). It wasobservedthatbotharti¢cialandnaturalfoodsareim-portant for the growth of rohu and common carp.Thepondstudyshowedsynergisticeiectsonnaturalfood availability in rohu^common carp polyculture.The question was raised as to whether the observedsynergism was mainly caused by increased naturalfoodavailabilityorbyachange in feedingbehaviour.Behaviouralstudiesinpondsaredi⁄cultduetohighturbidityand, consequently, low visibility.Therefore,to be able to observe the feeding behaviour, pondtreatments were simulated in aquaria.The eiects ofarti¢cial feed applicationand diierent stocking den-sities of common carp (0, 1 and 2tank

This study sought to examine the role of bile acids in the regulation of glucose and lipid metabolism, intestinal flora, and growth in high-fat diet-fed common carp (Cyprinus carpio L.). Fish (6.34 ± 0.07 g) were fed for 56 days with three different diets, the control diet (CO, 5.4% lipid), high-fat diet (HF, 11% lipid), and high-fat diet with 60 mg/kg bile acids (BAs, 11% lipid). The results showed that high-fat diets resulted in poor growth performance and increased triglyceride (TG) in serum and the liver. The addition of bile acids significantly alleviated the adverse effects of a high-fat diet. The mRNA expression results indicated that bile acids may improve lipid metabolism through the enhancement of the peroxisome proliferator-activated receptor (PPARa). The expression of gluconeogenesis-related phosphoenolpyruvate carboxykinase (PEPCK) mRNA was inhibited, while fibroblast growth factor 19 (FGF19) was significantly higher. Bile acids reshaped the intestinal microflora community, with the level of Bacteroidetes increasing. The correlation analysis indicated that Patescibacteria, Dependentiae, Myxococcota, and Planctomycetota in the gut are associated with genes involved in glucose and lipid metabolism. These results indicated that bile acids could ameliorate the negative effects of high-fat diets on common carp.

Innate immune response against an oomycete pathogen Aphanomyces invadans in common carp (Cyprinus carpio), a fish resistant to epizootic ulcerative syndrome

Molecular characterizations, immune modulation, and antibacterial activity of interleukin-17A/F1a and interleukin-17A/F1b in common carp Cyprinus carpio

Growth performance and immune status in common carp Cyprinus carpio as affected by plant oil-based diets complemented with β-glucan