Abstract
To investigate the regulation of the LH hCG receptor gene by gonadotropins, we examined the effect of PMSG and hCG on the expression of LH hCG receptor in immature rat ovary. Northern blot analysis of ovarian RNA revealed a major mRNA of 5400 nucleotides and minor species of 7500, 3600, 2300 and 1200 nucleotides, and PMSG treatment slightly increased the intensity of all LH hCG receptor messengers. Subsequently, hCG treatment decreased the number of LH hCG receptor by day 2 and mRNA levels by 12h after injection. The level of mRNA recovered and increased 5-fold of control by day 6, then returned to control levels by day 10, followed by slower decline in LH hCG receptor in plasma membrane. These studies demonstrate that the effects of PMSG and hCG on the number of LH hCG receptor are closely related to the actions of these hormones on LH hCG receptor messenger levels.
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