Regulation of Escherichia coli Polynucleotide Phosphorylase by ATP

Marta Del Favero,Elisa Mazzantini,Federica Briani,Sandro Zangrossi,Paolo Tortora,Gianni Dehò

doi:10.1074/jbc.c800113200

Marta Del Favero, Elisa Mazzantini + Show 4 more

Open Access

https://doi.org/10.1074/jbc.c800113200

Copy DOI

Abstract

Polynucleotide phosphorylase (PNPase), an enzyme conserved in bacteria and eukaryotic organelles, processively catalyzes the phosphorolysis of RNA, releasing nucleotide diphosphates, and the reverse polymerization reaction. In Escherichia coli, both reactions are implicated in RNA decay, as addition of either poly(A) or heteropolymeric tails targets RNA to degradation. PNPase may also be associated with the RNA degradosome, a heteromultimeric protein machine that can degrade highly structured RNA. Here, we report that ATP binds to PNPase and allosterically inhibits both its phosphorolytic and polymerization activities. Our data suggest that PNPase-dependent RNA tailing and degradation occur mainly at low ATP concentrations, whereas other enzymes may play a more significant role at high energy charge. These findings connect RNA turnover with the energy charge of the cell and highlight unforeseen metabolic roles of PNPase.

Highlights

Polynucleotide phosphorylase (PNPase),3 a polyribonucleotide nucleotidyltransferase (EC 2.7.7.8), is a homotrimeric enzyme involved in RNA turnover in bacteria and eukaryotic organelles [1]
PNPase was originally implicated in the synthesis of cellular RNA before the template-dependent RNA polymerase was discovered [2, 15, 16]; later on, because of its phosphorolytic activity, it was implicated in RNA degradation [6]
Because in Escherichia coli PNPase-dependent heteropolymeric failing, like polyadenylation by polyadenyl polymerase (PAP), targets bacterial RNAs to degradation [20], both PNPase phosphorolytic and polymerization activities participate in PNPase-dependent RNA decay

Summary

Introduction

Polynucleotide phosphorylase (PNPase),3 a polyribonucleotide nucleotidyltransferase (EC 2.7.7.8), is a homotrimeric enzyme involved in RNA turnover in bacteria and eukaryotic organelles [1]. We report that ATP binds to PNPase and allosterically inhibits both its phosphorolytic and polymerization activities.

Results

Conclusion