Regulation of dopamine production in the brains during sexual maturation in male honey bees

Abstract

To explore the physiological mechanisms that underlie age-related dopamine increases during sexual maturation in the brains of male honey bees, we focused on the expression of genes encoding the enzymes tyrosine hydroxylase (Amth) and DOPA decarboxylase (Amddc), which are involved in dopamine biosynthesis in the brain. We hypothesized that juvenile hormone in hemolymph and tyrosine intake from food known as factors enhancing brain dopamine levels might both control the expression of genes related to dopamine production, and we tested this hypothesis in experiments. The brain levels of tyrosine and DOPA, which are precursors of dopamine, decreased as males aged, whereas the dopamine levels increased, suggesting active metabolism of dopamine precursors. The relative expression levels of Amth and Amddc were significantly higher in the brains of 4-day-old males compared with 0-day-old males, and the higher level of Amddc was maintained after 8 days. Topical application of the juvenile hormone analog methoprene enhanced the expression levels of Amth and Amddc in the brains according to the methoprene concentration. Oral intake of tyrosine enhanced the tyrosine, DOPA and dopamine levels in the brain, and activated Amddc expression in the brain, suggesting that tyrosine intake can increase both substrates and enzyme for dopamine biosynthesis. These results support our hypothesis that juvenile hormone and tyrosine intake may enhance the expression levels of genes encoding enzymes involved in dopamine biosynthesis in male honey bee brains during sexual maturation.