Abstract
ABSTRACT : We characterized the one-electron reduction of oligodeoxynucleotides with a complementary ODN 6 in the X2-oxopropyl group on a thymine base (d oxo T) and applied the reaction to the radiolytic activation of DNAzyme function. We designed a system in which the DNAzyme function of cleaving mRNA was suppressed by introduction of d oxo T into the strand of DNAzyme. Figure 2 shows a representative profile for the radiolytic reduction of Hypoxic X-irradiation led to recovery of the cleavage ability because the 2-oxopropyl group was removed to form unmodified DNAzyme. We characterized the DNAzyme function by monitoring of which increased as a function of increasing radiation dose. To the fluorescence change of fluorophore- and quencher-labeled target strands. We confirmed that the DNAzyme function could be regulated by hypoxic X-irradiation and the reaction of d oxo T. Stimuli-responsive DNAs offer favorable properties for biological applications such as gene analysis and therapy.
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