Regulation of Ciap1 and Xiap Expression by Nitric Oxide, Cytokines, and Its Relation to Apotosis Induction in Rat Mesangial Cells and Raw 264.7 Macrophages.

Markus Manderscheid,Josef Pfeilschifter,Udo K Meszligmer

doi:10.1100/tsw.2001.143

Abstract

INTRODUCTION. Mesangial cells and RAW 264.7 macrophages respond to different NO donors within 16-24h or 6-8h, respectively, with apoptotic cell death (1). RAW 264.7 macrophages also die in response to endogenous NO production. In contrast, endogenous NO production failed to significantly induce cell death in mesangial cells (2,3). We hypothesized that differences in the expression of antiapoptotic proteins in particular the inhibitor of apoptosis (IAP) protein family might be responsible for this cell type-specific behaviour.

Highlights

Mesangial cells and RAW 264.7 macrophages respond to different NO donors within 16-24h or 6-8h, respectively, with apoptotic cell death (1)
XIAP mRNA levels remained unaffected by cytokines
Coincubation of cells with IL-1β and TNF-α or IL-1β and bFGF resulted in a synergistic induction of iNOS, comparable potentiating effects were absent regarding cIAP1 induction

Summary

Introduction

Mesangial cells and RAW 264.7 macrophages respond to different NO donors within 16-24h or 6-8h, respectively, with apoptotic cell death (1). In mesangial cells IL-1β and TNF-α induced cIAP1 mRNA expression within 3 h.

Results

Conclusion