Regulation of carbon flow through the glyoxylate and tricarboxylic acid cycles in the mitochondria of Turbatrix aceti—I. Coarse and fine controls

Abstract

1. 1. Subfractionation of Turbatrix aceti mitochondria showed that key gluconeogenic-related enzymes were associated with either the mitochondrial inner membrane or the matrix, not the outer membrane or intermembrane space. 2. 2. Conditions were established for sustained growth of Turbatrix aceti in media with varied carbon sources. 3. 3. Worms grown in the various media exhibited differences in growth rate, lipid content, enzyme specific activity, K m values, and electrophoretic patterns of multiple-form enzymes indicating that coarse control mechanisms operate to regulate carbon flow through the tricarboxylic acid and glyoxylate cycles. 4. 4. Differential effects of metabolites on isocitrate-metabolizing enzymes indicated that rapid, short-term responses of enzymes (fine control mechanisms) are also involved in controlling carbon flux through both cycles.