Regulation of calcium signalling by 1-O-alkylglycerols in human Jurkat T lymphocytes

Abstract

We studied the role of natural occurring 1-O-alkylglycerols on the calcium signalling in Jurkat T-cells. Alkylglycerols evoked an increase in free intracellular calcium concentration [Ca 2+]i, in a dose-dependent manner. When the experiments were performed in calcium-free buffer, the alkylglycerol response on the rise of [Ca 2+]i was wholly abolished compared with the one in calcium-containing buffer, suggesting that these etherlipids induce a calcium influx by the opening of Ca 2+ channels. We further employed inhibitors of voltage-gated calcium channels. We observed that ω-conotoxin, a blocker of N-type voltage-activated Ca 2+ channels, but not verapamil, a blocker of L-type voltage-activated Ca 2+ channels, curtailed significantly the calcium rise evoked by the lipid agents. Alkylglycerols also induced plasma membrane depolarisation, known to be involved in the opening of the voltage-gated calcium channels. Our study shows that alkylglycerols increase [Ca 2+]i influx in human Jurkat T-cells possibly by modulating the permeability of calcium channels.