Abstract
We investigated brain-specific transcription elements in the distal region (−253 to −54) of the mouse myelin basic protein (MBP) promoter by in vitro transcription using mouse brain nuclear extracts. Using deletion and base substitution mutants, we identified one tissue-specific transcription element at the downstream core of the NFI-site between −115 and −111. Footprinting assay demonstrated that three transcription factors bind around this element; NFI-related (−130 to −111), M1 (−110 to −97) and Sp1 (−92 to −84). NFI-related factor(s) in brain extracts consisted of a characteristic population which was different from that in other tissues. Methylation interference experiments revealed that this factor(s) interacted with the NFI-downstream core in a brain-specific manner. We suggest the existence of a brain-specific NFI-related factor(s).
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