Regulation of Bilirubin Clearance by Ligand-Activated Transcription Factors of the Endo- and Xenobiotic Metabolism System

Abstract

Bilirubin, the product of heme catabolism, represents an important endobiotic of the endo-and xenobiotic metabolism system including drug-metabolizing enzymes, drug transporters, and drug-inducible ligand-activated transcription factors (LATFs). Bilirubin is of clinical concern since severe neonatal jaundice may lead to “kernicterus” and neurotoxicity (Kapitulnik, 2004). Hyperbilirubinemia in the newborn is particularly a problem in preterm infants owing to more profound hemolysis, and immaturity of liver function. However, bilirubin is also an interesting antioxidant which may attenuate atherosclerosis (Lin et al., 2008). Hence, bilirubin synthesis and its catabolism has to be strictly controlled. The key to bilirubin's elimination is glucuronidation by UDP-glucuronosyltransferase UGT1A1. However, alternative pathways of bilirubin catabolism have also been observed. For example, the dioxin TCDD, a “classical” ligand of the Ah receptor (AhR) stimulates bilirubin elimination in congenitally jaundiced, UGT1A1-defective Gunn rats (Kapitulnik and Ostrow, 1977). Furthermore, bilirubin itself activates the AhR postnatally, leading to increased transcription of CYP1A1 and CYP1A2 in Gunn rats (Kapitulnik and Gonzalez, 1993). Bilirubin and its extrahepatically formed glucuronides are taken up from blood into hepatocytes by OATP1B1 and OATP1B3 (Nies et al., 2008). Following conjugation bilirubin glucuronides are biliary secreted by ABCC2. UGT1A1 and all the above transporters are transcriptionally regulated by the constitutive androstane receptor CAR (Huang et al., 2003). In CAR-defective mice phenobarbital-mediated induction of these proteins is absent. When human CAR is transfected into CAR-null mice phenobarbital and bilirubin itself activate CAR, the latter finding suggesting homeostatic control between enzyme substrate and activation of its major transcription factor. Bilirubin is a ligand of the AhR, a multifunctional transcription factor which induces a different set of genes than CAR in the drug metabolism system (Nguyen and Bradfield, 2008). It was discovered by the induction of aryl hydrocarbon metabolism and as mediator of dioxin toxicity. However, the AhR is also involved in development, cell proliferation, differentiation, and immune biology. The importance of the AhR in UGT1A1 induction has been demonstrated in human hepatocyte cultures treated with 3-methylcholanthrene (Ritter et al., 1999). Notably, phenobarbital and bilirubin are not ligands of CAR but activate CAR via phosphorylation/dephosphorylation pathways (Timsit and Negishi, 2007). Binding sites for CAR and the AhR have been identified in a 290-bp cluster of the human UGT1A1, termed gtPBREM (phenobarbital responsive enhancer module; Figure ​Figure1;1; Sugatani et al., 2005). The gtPBREM cluster contains binding sites for a number of ligand-activated transcription factors including pregnane X receptor (PXR), glucocorticoid receptors GR1 and GR2, CAR, antioxidant-responsive Nrf2 (Yueh and Tukey, 2007), AhR, and fibrate-inducible peroxisome proliferator-activated receptor PPARα (Seneko-Effenberger et al., 2007). It is important to note that the CAR binding site (gtNR1) plays a central role in UGT1A1 induction by both CAR and PXR, which is supported by site-directed mutagenesis and electrophoretic mobility shift assays (Sugatani et al., 2005). The cluster appears to be evolutionary conserved since the same arrangement of LATF-binding sites was found in the baboon (Caspersen et al., 2007). Allelic variants involved in bilirubin clearance have also been detected in the gtPBREM cluster. For example, the (TA)7 promoter polymorphism UGT1A1 *28, responsible for Gilbert's syndrome, is often in linkage disequilibrium with the T3279G polymorphism in the gtPBREM cluster (Sugatani et al., 2008). Figure 1 Schematic illustration of allelic variants and the position of response elements binding ligand-activated transcription factors in the enhancer/promoter region of human UGT1A1. Positions are taken from Sugatani et al. (2005), and Bock and Kohle ...