Abstract
Objective: To investigate the role of TIP30 in the apoptotic signal pathway in HepG2, and Hep3B and Hu-7 hepatoblastoma cell lines. Methods: In order to confirm whether TIP30 conducted Bcl-2 family was involved in apoptosis signal pathway, MTT assay, in situ 3’ end labelling of DNA assay and Western blot were carried out to detect the diverse apoptotic function of TIP30 and the regulation of Bcl-2 family. Results: TIP30 induced apoptosis as evidenced by morphological changes in hepatoblastoma cells, which was accompanied by up-regulating Bax and Bad proteins and stimulating them from cytoplasm to mitochondria, and down-regulating Bcl-xl, while it had no effect on the level of Bak protein. Conclusion: TIP30 induced apoptosis partly by modulating the protein levels of members of Bcl-2 family in hepatoblastoma cells. Elucidating the mechanism by which TIP30 induces cell death might establish it as an anticancer factor.
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